Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication.
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ABSTRACT:
SUBMITTER: Elazar M
PROVIDER: S-EPMC154017 | biostudies-literature | 2003 May
REPOSITORIES: biostudies-literature
Publications
Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication.
Elazar Menashe M Cheong Kwang Ho KH Liu Ping P Greenberg Harry B HB Rice Charles M CM Glenn Jeffrey S JS
Journal of virology 20030501 10
We identified an N-terminal amphipathic helix (AH) in one of hepatitis C virus (HCV)'s nonstructural proteins, NS5A. This AH is necessary and sufficient for membrane localization and is conserved across isolates. Genetically disrupting the AH impairs HCV replication. Moreover, an AH peptide-mimic inhibits the membrane association of NS5A in a dose-dependent manner. These results have exciting implications for the HCV life cycle and novel antiviral strategies. ...[more]
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