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Characterization of the chlorate reductase from Pseudomonas chloritidismutans.


ABSTRACT: A chlorate reductase has been purified from the chlorate-reducing strain Pseudomonas chloritidismutans. Comparison with the periplasmic (per)chlorate reductase of strain GR-1 showed that the cytoplasmic chlorate reductase of P. chloritidismutans reduced only chlorate and bromate. Differences were also found in N-terminal sequences, molecular weight, and subunit composition. Metal analysis and electron paramagnetic resonance measurements showed the presence of iron and molybdenum, which are also found in other dissimilatory oxyanion reductases.

SUBMITTER: Wolterink AF 

PROVIDER: S-EPMC154085 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature

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Characterization of the chlorate reductase from Pseudomonas chloritidismutans.

Wolterink Arthur F W M AF   Schiltz Emile E   Hagedoorn Peter-Leon PL   Hagen Wilfred R WR   Kengen Servé W M SW   Stams Alfons J M AJ  

Journal of bacteriology 20030501 10


A chlorate reductase has been purified from the chlorate-reducing strain Pseudomonas chloritidismutans. Comparison with the periplasmic (per)chlorate reductase of strain GR-1 showed that the cytoplasmic chlorate reductase of P. chloritidismutans reduced only chlorate and bromate. Differences were also found in N-terminal sequences, molecular weight, and subunit composition. Metal analysis and electron paramagnetic resonance measurements showed the presence of iron and molybdenum, which are also  ...[more]

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