Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls.
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ABSTRACT:
SUBMITTER: Bauer S
PROVIDER: S-EPMC1544100 | biostudies-literature | 2006 Jul
REPOSITORIES: biostudies-literature
Publications
Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls.
Bauer Stefan S Vasu Prasanna P Persson Staffan S Mort Andrew J AJ Somerville Chris R CR
Proceedings of the National Academy of Sciences of the United States of America 20060714 30
To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 ...[more]
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