WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells.
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ABSTRACT:
SUBMITTER: Anselmo AN
PROVIDER: S-EPMC1544143 | biostudies-literature | 2006 Jul
REPOSITORIES: biostudies-literature
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WNK1 and OSR1 regulate the Na+, K+, 2Cl- cotransporter in HeLa cells.
Anselmo Anthony N AN Earnest Svetlana S Chen Wei W Juang Yu-Chi YC Kim Sung Chan SC Zhao Yingming Y Cobb Melanie H MH
Proceedings of the National Academy of Sciences of the United States of America 20060710 29
Oxidative stress-responsive kinase (OSR) 1 and sterile20-related, proline-, alanine-rich kinase (SPAK) are Ste20p-related protein kinases that bind to the sodium, potassium, two chloride cotransporter, NKCC. Here we present evidence that the protein kinase with no lysine [K] (WNK) 1 regulates OSR1, SPAK, and NKCC activities. OSR1 exists in a complex with WNK1 in cells, is activated by recombinant WNK1 in vitro, and is phosphorylated in a WNK1-dependent manner in cells. Depletion of WNK1 from HeL ...[more]
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