Ontology highlight
ABSTRACT:
SUBMITTER: Narberhaus F
PROVIDER: S-EPMC154415 | biostudies-literature | 2003 May
REPOSITORIES: biostudies-literature
Journal of bacteriology 20030501 9
The sigma factor RpoH (sigma(32)) is the key regulator of the heat shock response in Escherichia coli. Many structural and functional properties of the sigma factor are poorly understood. To gain further insight into RpoH regions that are either important or dispensable for its cellular activity, we generated a collection of tetrapeptide insertion variants by a recently established in vitro linker insertion mutagenesis technique. Thirty-one distinct insertions were obtained, and their sigma fact ...[more]