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Effect of signal compounds and incubation conditions on the culturability of freshwater bacterioplankton.


ABSTRACT: The effect of signal compounds and of different incubation conditions on the culturability (i.e., the fraction of all cells capable of growth) of natural bacterioplankton from the eutrophic lake Zwischenahner Meer was investigated over a period of 20 months. Numbers of growing cells were determined by the most-probable-number technique in liquid media containing low concentrations (10 micro M) of the signal compounds N-(oxohexanoyl)-DL-homoserine lactone, N-(butyryl)-DL-homoserine lactone, cyclic AMP (cAMP), or ATP. cAMP was the most effective signal compound, leading to significantly increased cultivation efficiencies of up to 10% of the total bacterial counts. Microautoradiography with [2,8-(3)H]cAMP, combined with fluorescence in situ hybridization, demonstrated that cAMP was taken up by 18% of all cells. The bacterial cAMP uptake systems had a very low K(m) value of

SUBMITTER: Bruns A 

PROVIDER: S-EPMC154826 | biostudies-literature | 2003 Apr

REPOSITORIES: biostudies-literature

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Effect of signal compounds and incubation conditions on the culturability of freshwater bacterioplankton.

Bruns Alke A   Nübel Ulrich U   Cypionka Heribert H   Overmann Jörg J  

Applied and environmental microbiology 20030401 4


The effect of signal compounds and of different incubation conditions on the culturability (i.e., the fraction of all cells capable of growth) of natural bacterioplankton from the eutrophic lake Zwischenahner Meer was investigated over a period of 20 months. Numbers of growing cells were determined by the most-probable-number technique in liquid media containing low concentrations (10 micro M) of the signal compounds N-(oxohexanoyl)-DL-homoserine lactone, N-(butyryl)-DL-homoserine lactone, cycli  ...[more]

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