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Dynamic assembly of surface structures in living cells.

ABSTRACT: Although the dynamics of cell membranes and associated structures is vital for cell function, little is known due to lack of suitable methods. We found, using scanning ion conductance microscopy, that microvilli, membrane projections supported by internal actin bundles, undergo a life cycle: fast height-dependent growth, relatively short steady state, and slow height-independent retraction. The microvilli can aggregate into relatively stable structures where the steady state is extended. We suggest that the intrinsic dynamics of microvilli, combined with their ability to make stable structures, allows them to act as elementary "building blocks" for the assembly of specialized structures on the cell surface.

SUBMITTER: Gorelik J

PROVIDER: S-EPMC156284 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature

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Dynamic assembly of surface structures in living cells.

Gorelik Julia J Shevchuk Andrew I AI Frolenkov Gregory I GI Diakonov Ivan A IA Lab Max J MJ Kros Corne J CJ Richardson Guy P GP Vodyanoy Igor I Edwards Christopher R W CR Klenerman David D Korchev Yuri E YE

Proceedings of the National Academy of Sciences of the United States of America 20030429 10

Although the dynamics of cell membranes and associated structures is vital for cell function, little is known due to lack of suitable methods. We found, using scanning ion conductance microscopy, that microvilli, membrane projections supported by internal actin bundles, undergo a life cycle: fast height-dependent growth, relatively short steady state, and slow height-independent retraction. The microvilli can aggregate into relatively stable structures where the steady state is extended. We sugg ...[more]

PMID: 12721367

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