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Specific interactions of distamycin with G-quadruplex DNA.

ABSTRACT: Distamycin binds the minor groove of duplex DNA at AT-rich regions and has been a valuable probe of protein interactions with double-stranded DNA. We find that distamycin can also inhibit protein interactions with G-quadruplex (G4) DNA, a stable four-stranded structure in which the repeating unit is a G-quartet. Using NMR, we show that distamycin binds specifically to G4 DNA, stacking on the terminal G-quartets and contacting the flanking bases. These results demonstrate the utility of distamycin as a probe of G4 DNA-protein interactions and show that there are (at least) two distinct modes of protein-G4 DNA recognition which can be distinguished by sensitivity to distamycin.

SUBMITTER: Cocco MJ

PROVIDER: S-EPMC156726 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature

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Specific interactions of distamycin with G-quadruplex DNA.

Cocco Melanie J MJ Hanakahi L A LA Huber Michael D MD Maizels Nancy N

Nucleic acids research 20030601 11

Distamycin binds the minor groove of duplex DNA at AT-rich regions and has been a valuable probe of protein interactions with double-stranded DNA. We find that distamycin can also inhibit protein interactions with G-quadruplex (G4) DNA, a stable four-stranded structure in which the repeating unit is a G-quartet. Using NMR, we show that distamycin binds specifically to G4 DNA, stacking on the terminal G-quartets and contacting the flanking bases. These results demonstrate the utility of distamyci ...[more]

PMID: 12771220

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