Ontology highlight
ABSTRACT:
SUBMITTER: Smart D
PROVIDER: S-EPMC1571834 | biostudies-literature | 2000 Jan
REPOSITORIES: biostudies-literature
Smart D D Gunthorpe M J MJ Jerman J C JC Nasir S S Gray J J Muir A I AI Chambers J K JK Randall A D AD Davis J B JB
British journal of pharmacology 20000101 2
The endogenous cannabinoid anandamide was identified as an agonist for the recombinant human VR1 (hVR1) by screening a large array of bioactive substances using a FLIPR-based calcium assay. Further electrophysiological studies showed that anandamide (10 or 100 microM) and capsaicin (1 microM) produced similar inward currents in hVR1 transfected, but not in parental, HEK293 cells. These currents were abolished by capsazepine (1 microM). In the FLIPR anandamide and capsaicin were full agonists at ...[more]