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Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand.

ABSTRACT: Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodents were found positive for the hantaviral S- and M-segment nucleotide sequences. Genetic analysis revealed that the four newly described wild-type strains belong to Thailand hantavirus. On the phylogenetic trees they formed a well-supported cluster within the group of Murinae-associated hantaviruses and shared a recent common ancestor with Seoul virus.

SUBMITTER: Hugot JP

PROVIDER: S-EPMC1578555 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature

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Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand.

Hugot Jean-Pierre JP Plyusnina Angelina A Herbreteau Vincent V Nemirov Kirill K Laakkonen Juha J Lundkvist Ake A Supputamongkol Yupin Y Henttonen Heikki H Plyusnin Alexander A

Virology journal 20060905

Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodent ...[more]

PMID: 16953877

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