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DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase.

ABSTRACT: CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA replication has not yet been addressed. Here we show that antibodies to the Xenopus laevis homolog, xCdc7, interfere with DNA replication in vivo in developing embryos and in vitro in cycling egg extracts. We also demonstrate cell cycle-dependent association of xCdc7 with the Mcm complex, which binds to replication origins and also is required for DNA synthesis. Taken together, these data indicate that the function of xCdc7 is conserved from fungi to vertebrates. xCdc7 protein accumulates after stimulation of resting oocytes with progesterone, suggesting a molecular explanation for previous observations that the development of the capacity for DNA replication requires protein synthesis late in meiosis I.

SUBMITTER: Roberts BT

PROVIDER: S-EPMC15849 | biostudies-literature | 1999 Mar

REPOSITORIES: biostudies-literature

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DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase.

Roberts B T BT Ying C Y CY Gautier J J Maller J L JL

Proceedings of the National Academy of Sciences of the United States of America 19990301 6

CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA replication has not yet been addressed. Here we show that antibodies to the Xenopus laevis homolog, xCdc7, interfere with DNA replication in vivo in developing embryos and in vitro in cycling egg extracts. We also demonstrate cell cycle-dependent association of xCdc7 with the Mcm complex, which binds to replication origins and also ...[more]

PMID: 10077591

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