Ontology highlight
ABSTRACT:
SUBMITTER: Gallup JM
PROVIDER: S-EPMC1592462 | biostudies-literature | 2006
REPOSITORIES: biostudies-literature
Biological procedures online 20060915
The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamically-sound, truly LOG-linear qPCR reactions very quickly. The qPCR problems this invention solves are universal to all qPCR reactions, and it performs all necessary qPCR set-up calculations in about 52 ...[more]