Ontology highlight
ABSTRACT:
SUBMITTER: Frederickson S
PROVIDER: S-EPMC1599960 | biostudies-literature | 2006 Sep
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20060914 39
By using rational design, antibody fragments (Fabs) that mimic thrombopoietin (TPO) were created. A peptide with cMpl receptor-binding capability was grafted into different complementarity-determining regions of a fully human Fab scaffold. Functional presentation of the peptide was optimized by using phage display and cell-based panning. Select antibodies and fragments containing two grafted peptides were assayed for their ability to stimulate the cMpl receptor in vitro. Several candidates demon ...[more]