Ontology highlight
ABSTRACT:
SUBMITTER: Kiraly O
PROVIDER: S-EPMC1604731 | biostudies-literature | 2006 Jul
REPOSITORIES: biostudies-literature
Király Orsolya O Guan Lan L Szepessy Edit E Tóth Miklós M Kukor Zoltán Z Sahin-Tóth Miklós M
Protein expression and purification 20060221 1
High-level expression of human trypsinogens as inclusion bodies in Escherichia coli requires deletion of the secretory signal sequence and placement of an initiator methionine at the N terminus. Trypsinogen preparations obtained this way contain a mixture of abnormal N termini, as a result of processing by cytoplasmic aminopeptidases. Here, we describe an expression system that produces recombinant human cationic trypsinogen with a native, intact N terminus, using intein-mediated protein splicin ...[more]