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Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella.


ABSTRACT: A novel cDNA clong encoding a cytochrome P450 was screened from the insecticide-susceptible strain of Plutella xylostella (L.) (Lepidoptera:Yponomeutidae). The nucleotide sequence of the clone, designated CYP6BF1, was determined. This is the first full-length sequence of the CYP6 family from Plutella xylostella (L.). The cDNA is 1661bp in length and contains an open reading frame from base pairs 26 to 1570, encoding a protein of 514 amino acid residues. It is similar to the other insect P450s in gene family 6, including CYP6AE1 from Depressaria pastinacella, (46%). The GenBank accession number is AY971374.

SUBMITTER: Li H 

PROVIDER: S-EPMC1615252 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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Molecular cloning and nucleotide sequence of CYP6BF1 from the diamondback moth, Plutella xylostella.

Li Hongshan H   Dai Huaguo H   Wei Hui H  

Journal of insect science (Online) 20051222


A novel cDNA clong encoding a cytochrome P450 was screened from the insecticide-susceptible strain of Plutella xylostella (L.) (Lepidoptera:Yponomeutidae). The nucleotide sequence of the clone, designated CYP6BF1, was determined. This is the first full-length sequence of the CYP6 family from Plutella xylostella (L.). The cDNA is 1661bp in length and contains an open reading frame from base pairs 26 to 1570, encoding a protein of 514 amino acid residues. It is similar to the other insect P450s in  ...[more]

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