Proteolytic activation of influenza viruses by serine proteases TMPRSS2 and HAT from human airway epithelium.
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ABSTRACT:
SUBMITTER: Bottcher E
PROVIDER: S-EPMC1617224 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Publications
Proteolytic activation of influenza viruses by serine proteases TMPRSS2 and HAT from human airway epithelium.
Böttcher Eva E Matrosovich Tatyana T Beyerle Michaela M Klenk Hans-Dieter HD Garten Wolfgang W Matrosovich Mikhail M
Journal of virology 20061001 19
Host cell proteases that cleave the hemagglutinin (HA) of influenza viruses in the human respiratory tract are still not identified. Here we cloned two human type II transmembrane serine proteases with known airway localization, TMPRSS2 and HAT, into mammalian expression vector. Cotransfection of mammalian cells with plasmids encoding HA and either protease resulted in HA cleavage in situ. Transient expression of either protease in MDCK cells enabled multicycle replication of influenza viruses i ...[more]
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