Ontology highlight
ABSTRACT:
SUBMITTER: Jiang J
PROVIDER: S-EPMC16185 | biostudies-literature | 2000 Mar
REPOSITORIES: biostudies-literature
Jiang J J Horowitz D S DS Xu R M RM
Proceedings of the National Academy of Sciences of the United States of America 20000301 7
The splicing factor Prp18 is required for the second step of pre-mRNA splicing. We have isolated and determined the crystal structure of a large fragment of the Saccharomyces cerevisiae Prp18 that lacks the N-terminal 79 amino acids. This fragment, called Prp18Delta79, is fully active in yeast splicing in vitro and includes the sequences of Prp18 that have been evolutionarily conserved. The core structure of Prp18Delta79 is compact and globular, consisting of five alpha-helices that adopt a nove ...[more]