Unknown

Dataset Information

0

An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locus.


ABSTRACT: The Gnas locus in distal mouse chromosome (Chr) 2 is emerging as a complex genomic region. It contains three imprinted genes in the order Nesp-Gnasxl-Gnas. Gnas encodes a G protein alpha-subunit, and Nesp and Gnasxl encode proteins of unknown function expressed in neuroendocrine tissue. Together, these genes form a single transcription unit because transcripts of Nesp and Gnasxl are alternatively spliced onto exon 2 of Gnas. Nesp and Gnasxl are expressed from opposite parental alleles, with Nesp encoding a maternal-specific transcript and Gnasxl encoding a paternal-specific transcript. We now identify a further imprinted transcript in this cluster. Reverse transcription-PCR analysis of Nesp expression in 15. 5-days-postcoitum embryos carrying only maternal or paternal copies of distal Chr 2 revealed an isoform that is exclusively paternally, rather than maternally, expressed. Strand-specific reverse transcription-PCR showed that this form is an antisense transcript. The existence of a paternally expressed antisense transcript was confirmed by Northern blot analysis. The sequence is contiguous with genomic sequence downstream of Nesp and encompasses Nesp exons 1 and 2 and an intervening intron. We propose that Nespas is an additional control element in the imprinting region of mouse distal Chr 2; it adds further complexity to the Gnas-imprinted gene cluster.

SUBMITTER: Wroe SF 

PROVIDER: S-EPMC16241 | biostudies-literature | 2000 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

An imprinted transcript, antisense to Nesp, adds complexity to the cluster of imprinted genes at the mouse Gnas locus.

Wroe S F SF   Kelsey G G   Skinner J A JA   Bodle D D   Ball S T ST   Beechey C V CV   Peters J J   Williamson C M CM  

Proceedings of the National Academy of Sciences of the United States of America 20000301 7


The Gnas locus in distal mouse chromosome (Chr) 2 is emerging as a complex genomic region. It contains three imprinted genes in the order Nesp-Gnasxl-Gnas. Gnas encodes a G protein alpha-subunit, and Nesp and Gnasxl encode proteins of unknown function expressed in neuroendocrine tissue. Together, these genes form a single transcription unit because transcripts of Nesp and Gnasxl are alternatively spliced onto exon 2 of Gnas. Nesp and Gnasxl are expressed from opposite parental alleles, with Nesp  ...[more]

Similar Datasets

| S-EPMC403675 | biostudies-literature
| S-EPMC3063750 | biostudies-literature
| S-EPMC22380 | biostudies-literature
| S-EPMC5932550 | biostudies-literature
| S-EPMC3688811 | biostudies-literature
| S-EPMC7017182 | biostudies-literature
| S-EPMC4319783 | biostudies-literature
| S-EPMC2913043 | biostudies-literature
| S-EPMC166348 | biostudies-literature
| S-EPMC2671723 | biostudies-literature