Ontology highlight
ABSTRACT:
SUBMITTER: Bao S
PROVIDER: S-EPMC1624905 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
RNA (New York, N.Y.) 20060927 11
Double-stranded RNA (dsRNA) can induce post-transcriptional gene silencing in a wide variety of organisms. Commonly, inverted repeats are used to produce dsRNA to silence genes of interest. However, cloning inverted repeats still remains a rate-limiting step for widely applying this technique. Here we describe a pGEM-T-based vector, pGEM-WIZ, designed to produce inverted repeats for any Drosophila gene. pGEM-WIZ has a high efficiency in assembling inverted repeats and the repeats in this vector ...[more]