Ontology highlight
ABSTRACT:
SUBMITTER: Lee JH
PROVIDER: S-EPMC16334 | biostudies-literature | 1999 Apr
REPOSITORIES: biostudies-literature
Lee J H JH Choi S K SK Roll-Mecak A A Burley S K SK Dever T E TE
Proceedings of the National Academy of Sciences of the United States of America 19990401 8
Binding of initiator methionyl-tRNA to ribosomes is catalyzed in prokaryotes by initiation factor (IF) IF2 and in eukaryotes by eIF2. The discovery of both IF2 and eIF2 homologs in yeast and archaea suggested that these microbes possess an evolutionarily intermediate protein synthesis apparatus. We describe the identification of a human IF2 homolog, and we demonstrate by using in vivo and in vitro assays that human IF2 functions as a translation factor. In addition, we show that archaea IF2 can ...[more]