Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.
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ABSTRACT:
SUBMITTER: Klijn A
PROVIDER: S-EPMC1636199 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
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Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.
Klijn A A Moine D D Delley M M Mercenier A A Arigoni F F Pridmore R D RD
Applied and environmental microbiology 20060922 11
In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters. ...[more]
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