Ontology highlight
ABSTRACT:
SUBMITTER: Flodell S
PROVIDER: S-EPMC1636360 | biostudies-literature | 2006
REPOSITORIES: biostudies-literature
Flodell Sara S Petersen Michael M Girard Frederic F Zdunek Janusz J Kidd-Ljunggren Karin K Schleucher Jürgen J Wijmenga Sybren S
Nucleic acids research 20060831 16
Hepatitis B virus (HBV) replication is initiated by HBV RT binding to the highly conserved encapsidation signal, epsilon, at the 5' end of the RNA pregenome. Epsilon contains an apical stem-loop, whose residues are either totally conserved or show rare non-disruptive mutations. Here we present the structure of the apical stem-loop based on NOE, RDC and (1)H chemical shift NMR data. The (1)H chemical shifts proved to be crucial to define the loop conformation. The loop sequence 5'-CUGUGC-3' folds ...[more]