Ontology highlight
ABSTRACT:
SUBMITTER: Kutyavin IV
PROVIDER: S-EPMC1636472 | biostudies-literature | 2006
REPOSITORIES: biostudies-literature
Kutyavin Igor V IV Milesi Dave D Belousov Yevgeniy Y Podyminogin Mikhail M Vorobiev Alexei A Gorn Vladimir V Lukhtanov Eugeny A EA Vermeulen Nicolaas M J NM Mahoney Walt W
Nucleic acids research 20060929 19
Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substrate that mimics the abasic lesions that normally occur in double-stranded DNA. The three component substrate is characterized by single-stranded DNA target, an oligonucleotide probe, separated from a helper oligonucleotide by a one base gap. The oligonucleotide probe contains a non-fluorescent quencher at the 5' end and fluorophore attached to the 3' end through a special rigid linker. Fluorescence of the oligonucle ...[more]