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Tetraspanin CD151 regulates alpha6beta1 integrin adhesion strengthening.

ABSTRACT: The tetraspanin CD151 molecule associates specifically with laminin-binding integrins, including alpha6beta1. To probe strength of alpha6beta1-dependent adhesion to laminin-1, defined forces (0-1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin-1, and detachment of beads coated with fibronectin or anti-alpha6 antibody were all unaffected by CD151 mutation. Hence, CD151 plays a key role in selectively strengthening alpha6beta1 integrin-mediated adhesion to laminin-1.

SUBMITTER: Lammerding J

PROVIDER: S-EPMC164635 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature

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Tetraspanin CD151 regulates alpha6beta1 integrin adhesion strengthening.

Lammerding Jan J Kazarov Alexander R AR Huang Hayden H Lee Richard T RT Hemler Martin E ME

Proceedings of the National Academy of Sciences of the United States of America 20030612 13

The tetraspanin CD151 molecule associates specifically with laminin-binding integrins, including alpha6beta1. To probe strength of alpha6beta1-dependent adhesion to laminin-1, defined forces (0-1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal region replaced) showed impaired adhesion st ...[more]

PMID: 12805567

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