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Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum.


ABSTRACT: A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.

SUBMITTER: Okamura Y 

PROVIDER: S-EPMC165158 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature

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Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum.

Okamura Yoshiko Y   Takeyama Haruko H   Sekine Takumi T   Sakaguchi Toshifumi T   Wahyudi Aris Tri AT   Sato Rika R   Kamiya Shinji S   Matsunaga Tadashi T  

Applied and environmental microbiology 20030701 7


A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed. ...[more]

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