Ontology highlight
ABSTRACT:
SUBMITTER: Kato S
PROVIDER: S-EPMC166245 | biostudies-literature | 2003 Jul
REPOSITORIES: biostudies-literature
Kato Shunsuke S Han Shuang-Yin SY Liu Wen W Otsuka Kazunori K Shibata Hiroyuki H Kanamaru Ryunosuke R Ishioka Chikashi C
Proceedings of the National Academy of Sciences of the United States of America 20030625 14
Inactivation of the tumor suppressor p53 by missense mutations is the most frequent genetic alteration in human cancers. The common missense mutations in the TP53 gene disrupt the ability of p53 to bind to DNA and consequently to transactivate downstream genes. However, it is still not fully understood how a large number of the remaining mutations affect p53 structure and function. Here, we used a comprehensive site-directed mutagenesis technique and a yeast-based functional assay to construct, ...[more]