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A functional genomics approach toward the understanding of secondary metabolism in plant cells.

ABSTRACT: Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fragment length polymorphism-based transcript profiling of jasmonate-elicited tobacco Bright yellow 2 cells. Transcriptome analysis suggested an extensive jasmonate-mediated genetic reprogramming of metabolism, which correlated well with the observed shifts in the biosynthesis of the metabolites investigated. This method, which in addition to transcriptome data also generates gene tags, in the future might lead to the creation of novel tools for metabolic engineering of medicinal plant systems in general.

SUBMITTER: Goossens A 

PROVIDER: S-EPMC166274 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature

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A functional genomics approach toward the understanding of secondary metabolism in plant cells.

Goossens Alain A   Häkkinen Suvi T ST   Laakso Into I   Seppänen-Laakso Tuulikki T   Biondi Stefania S   De Sutter Valerie V   Lammertyn Freya F   Nuutila Anna Maria AM   Söderlund Hans H   Zabeau Marc M   Inzé Dirk D   Oksman-Caldentey Kirsi-Marja KM  

Proceedings of the National Academy of Sciences of the United States of America 20030625 14

Despite the tremendous importance of secondary metabolites for humans as for the plant itself, plant secondary metabolism remains poorly characterized. Here, we present an experimental approach, based on functional genomics, to facilitate gene discovery in plant secondary metabolism. Targeted metabolite analysis was combined with cDNA-amplified fragment length polymorphism-based transcript profiling of jasmonate-elicited tobacco Bright yellow 2 cells. Transcriptome analysis suggested an extensiv  ...[more]

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