Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism.

ABSTRACT:

Background

Although understanding of physiological interactions between plasmid DNA and its host is important for vector design and host optimization in many biotechnological applications, to our knowledge, global studies on plasmid-host interactions have not been performed to date even for well-characterized plasmids.

Results

Escherichia coli cells, either devoid of plasmid DNA or bearing plasmid pOri1 (with a single ColE1 replication origin) or plasmid pOri2 (with double ColE1 replication origins), were cultured in a chemostat. We used a combination of metabolic flux analysis, DNA microarray and enzyme activity analysis methods to explore differences in the metabolism between these strains. We found that the presence of plasmids significantly influenced various metabolic p

SUBMITTER: Wang Z 

PROVIDER: S-EPMC1664580 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature