Increased frequency of homologous recombination and T-DNA integration in Arabidopsis CAF-1 mutants.
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ABSTRACT:
SUBMITTER: Endo M
PROVIDER: S-EPMC1679757 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
Publications
Increased frequency of homologous recombination and T-DNA integration in Arabidopsis CAF-1 mutants.
Endo Masaki M Ishikawa Yuichi Y Osakabe Keishi K Nakayama Shigeki S Kaya Hidetaka H Araki Takashi T Shibahara Kei-ichi K Abe Kiyomi K Ichikawa Hiroaki H Valentine Lisa L Hohn Barbara B Toki Seiichi S
The EMBO journal 20061116 23
Chromatin assembly factor 1 (CAF-1) is involved in nucleo some assembly following DNA replication and nucleotide excision repair. In Arabidopsis thaliana, the three CAF-1 subunits are encoded by FAS1, FAS2 and, most likely, MSI1, respectively. In this study, we asked whether genomic stability is altered in fas1 and fas2 mutants that are lacking CAF-1 activity. Depletion of either subunit increased the frequency of somatic homologous recombination (HR) in planta approximately 40-fold. The frequen ...[more]
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