Ontology highlight
ABSTRACT:
SUBMITTER: Wozniak MJ
PROVIDER: S-EPMC1679764 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
Woźniak Marcin J MJ Allan Victoria J VJ
The EMBO journal 20061109 23
Kinesin-1 drives the movement of diverse cargoes, and it has been proposed that specific kinesin light chain (KLC) isoforms target kinesin-1 to these different structures. Here, we test this hypothesis using two in vitro motility assays, which reconstitute the movement of rough endoplasmic reticulum (RER) and vesicles present in a Golgi membrane fraction. We generated GST-tagged fusion proteins of KLC1B and KLC1D that included the tetratricopeptide repeat domain and the variable C-terminus. We f ...[more]