Ontology highlight
ABSTRACT:
SUBMITTER: Arenskotter M
PROVIDER: S-EPMC169124 | biostudies-literature | 2003 Aug
REPOSITORIES: biostudies-literature
Arenskötter Matthias M Baumeister Dirk D Kalscheuer Rainer R Steinbüchel Alexander A
Applied and environmental microbiology 20030801 8
Gene transfer systems for Gordonia polyisoprenivorans strains VH2 and Y2K based on electroporation and conjugation, respectively, were established. Several parameters were optimized, resulting in transformation efficiencies of >4 x 10(5) CFU/ micro g of plasmid DNA. In contrast to most previously described electroporation protocols, the highest efficiencies were obtained by applying a heat shock after the intrinsic electroporation. Under these conditions, transfer and autonomous replication of p ...[more]