FAPP2, cilium formation, and compartmentalization of the apical membrane in polarized Madin-Darby canine kidney (MDCK) cells.
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ABSTRACT:
SUBMITTER: Vieira OV
PROVIDER: S-EPMC1693701 | biostudies-literature | 2006 Dec
REPOSITORIES: biostudies-literature
Publications
FAPP2, cilium formation, and compartmentalization of the apical membrane in polarized Madin-Darby canine kidney (MDCK) cells.
Vieira Otilia V OV Gaus Katharina K Verkade Paul P Fullekrug Joachim J Vaz Winchil L C WL Simons Kai K
Proceedings of the National Academy of Sciences of the United States of America 20061120 49
We have analyzed the role of the phosphatidylinositol-4-phosphate adaptor protein-2 (FAPP2), a component of the apical transport machinery, in cilium formation in polarized Madin-Darby canine kidney (MDCK) cells. We show that ciliogenesis is defective in FAPP2 knockdown cells. Furthermore, by using fluorescence recovery after photobleaching studies of domain connectivity and the generalized polarization spectra of Laurdan, we demonstrate that FAPP2 depletion impairs the formation of condensed ap ...[more]
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