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Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae.

ABSTRACT: A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.

SUBMITTER: Ohkubo S

PROVIDER: S-EPMC1694208 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature

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Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae.

Ohkubo Satoshi S Miyashita Hideaki H Murakami Akio A Takeyama Haruko H Tsuchiya Tohru T Mimuro Mamoru M

Applied and environmental microbiology 20061006 12

A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae. ...[more]

PMID: 17028237

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