Transplacental RNAi: deciphering gene function in the postimplantation-staged embryo.
Ontology highlight
ABSTRACT:
SUBMITTER: O'shea KS
PROVIDER: S-EPMC1698258 | biostudies-literature | 2006
REPOSITORIES: biostudies-literature
Publications
Transplacental RNAi: deciphering gene function in the postimplantation-staged embryo.
O'shea K Sue KS De Boer Lisa S LS Slawny Nicole A NA Gratsch Theresa E TE
Journal of biomedicine & biotechnology 20060101 4
RNAi offers the opportunity to examine the role in postimplantation development of genes that cause preimplantation lethality and to create allelic series of targeted embryos. We have delivered constituitively expressed short hairpin (sh) RNAs to pregnant mice during the early postimplantation period of development and observed gene knockdown and defects that phenocopy the null embryo. We have silenced genes that have not yet been "knocked out" in the mouse (geminin and Wnt8b), those required du ...[more]
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