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Long-distance conformational changes in a protein engineered by modulated sequence duplication.

ABSTRACT: There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possible to introduce a mutation at one site in a protein that will induce large-scale changes in the structure at a spatially remote site.

SUBMITTER: Sagermann M

PROVIDER: S-EPMC170894 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature

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Long-distance conformational changes in a protein engineered by modulated sequence duplication.

Sagermann Martin M Gay Leslie L Matthews Brian W BW

Proceedings of the National Academy of Sciences of the United States of America 20030717 16

There are few, if any, known instances in which a biological signal is transmitted via a large conformational change through the body of a protein. We describe here a mutant of T4 lysozyme that was engineered to permit structural change at a distance. The design uses a tandem sequence repeat that makes it possible to transmit large-scale structural changes from one end of an alpha-helix to the other over a distance of 17-25 A. The method should be of general applicability and may make it possibl ...[more]

PMID: 12869697

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