Unknown

Dataset Information

0

Receptor-mediated endocytosis of soluble and membrane-tethered Sonic hedgehog by Patched-1.


ABSTRACT: Patched (Ptc) is the ligand-binding component of the Hedgehog (Hh) receptor complex. In the Drosophila embryo, Ptc and Hh colocalize in vesicular punctate structures. However, receptor-mediated endocytosis of Hh proteins has not been demonstrated. By using chick neural plate explants, we show that Sonic hedgehog (Shh)-responsive neural precursor cells internalize recombinant and endogenous Shh and provide direct evidence for a gradient of endogenous Shh in the ventral neural tube. Shh internalization is blocked by a monoclonal antibody whose epitope overlaps the Ptc-binding site of Shh. These findings suggest that Shh internalization is mediated by Ptc-1 and may be linked to signaling. Concordantly, transfection of mammalian cell lines with a Ptc-1 cDNA confers the ability to internalize multiple forms of Shh, including transmembrane-anchored Shh, by a dynamin-dependent process.

SUBMITTER: Incardona JP 

PROVIDER: S-EPMC17291 | biostudies-literature | 2000 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Receptor-mediated endocytosis of soluble and membrane-tethered Sonic hedgehog by Patched-1.

Incardona J P JP   Lee J H JH   Robertson C P CP   Enga K K   Kapur R P RP   Roelink H H  

Proceedings of the National Academy of Sciences of the United States of America 20001001 22


Patched (Ptc) is the ligand-binding component of the Hedgehog (Hh) receptor complex. In the Drosophila embryo, Ptc and Hh colocalize in vesicular punctate structures. However, receptor-mediated endocytosis of Hh proteins has not been demonstrated. By using chick neural plate explants, we show that Sonic hedgehog (Shh)-responsive neural precursor cells internalize recombinant and endogenous Shh and provide direct evidence for a gradient of endogenous Shh in the ventral neural tube. Shh internaliz  ...[more]

Similar Datasets

| S-EPMC7893488 | biostudies-literature
| S-EPMC6764859 | biostudies-literature
| S-EPMC5056095 | biostudies-literature
| S-EPMC1288546 | biostudies-literature
| S-EPMC6341490 | biostudies-literature
| S-EPMC2242683 | biostudies-literature
| S-EPMC6526471 | biostudies-literature
| S-EPMC5753398 | biostudies-other
| S-EPMC3169562 | biostudies-literature
| S-EPMC6326742 | biostudies-literature