Ontology highlight
ABSTRACT:
SUBMITTER: Braden BC
PROVIDER: S-EPMC17317 | biostudies-literature | 2000 Oct
REPOSITORIES: biostudies-literature
Braden B C BC Goldbaum F A FA Chen B X BX Kirschner A N AN Wilson S R SR Erlanger B F BF
Proceedings of the National Academy of Sciences of the United States of America 20001001 22
We have prepared a monoclonal Buckminsterfullerene specific antibody and report the sequences of its light and heavy chains. We also show, by x-ray crystallographic analysis of the Fab fragment and by model building, that the fullerene binding site is formed by the interface of the antibody light and heavy chains. Shape-complementary clustering of hydrophobic amino acids, several of which participate in putative stacking interactions with fullerene, form the binding site. Moreover, an induced fi ...[more]