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Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor.

ABSTRACT: Lymphatic vessels develop from specialized venous endothelial cells. Using knockout mice, we found that fasting-induced adipose factor (Fiaf) is required for functional partitioning of postnatal intestinal lymphatic and blood vessels. In wild-type animals, levels of intestinal Fiaf expression rise during the first postnatal day and peak at day 2, which coincides with the onset of the lymphatico-venous partitioning abnormality in Fiaf-/- mutants on a mixed 129/SvJ:C57BL/6 genetic background. Fiaf deficiency is not associated with disruption of the blood vasculature or with lymphatic endothelial recruitment of smooth muscle cells. We identified Prox1, a critical regulator of lymphangiogenesis, as a downstream target for Fiaf signaling in the intestinal lymphatic endothelium. This organ-specific lymphovascular abnormality can be rescued by allowing embryonic Fiaf-/- intestinal isografts to develop in Fiaf+/+ recipients.

SUBMITTER: Backhed F

PROVIDER: S-EPMC1761863 | biostudies-literature |

REPOSITORIES: biostudies-literature

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FOXC2 and fluid shear stress stabilize postnatal lymphatic vasculature.

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Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine.

Project description:The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion fixation, tissue sample preparation, immunofluorescence staining, and whole-mount preparation of stained samples. Our protocol will enable researchers to visualize and analyze the intricate network of vessels in the small intestine. For complete details on the use and execution of this protocol, please refer to Karaman et al. (2022).1.

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The blood vasculature instructs lymphatic patterning in a SOX7-dependent manner.

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Chemokine signaling directs trunk lymphatic network formation along the preexisting blood vasculature.

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| S-EPMC2148066 | biostudies-literature

The role of RNA interference in the developmental separation of blood and lymphatic vasculature.

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Project description:It was the purpose to analyse the changes in gene expression which occur in the mouse small intestine from the pre-weaning to the post-weaning stage. The gene expression was accordingly followed from postnatal day 4 to postnatal day 32. Experiment Overall Design: 6 time-points were defined corresponding to postnatal day 4, 7, 9, 11, 24 and 32. Ileal samples were taken from individual mice at each time-point. All layers of the small intestine were included in the sample. Three samples from individual mice were analysed by hybridization to Affymetrix GeneChips.

2008-06-16 | E-GEOD-8065 | biostudies-arrayexpress

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