Optimization of ribosome structure and function by rRNA base modification.

ABSTRACT:

Background

Translating mRNA sequences into functional proteins is a fundamental process necessary for the viability of organisms throughout all kingdoms of life. The ribosome carries out this process with a delicate balance between speed and accuracy. This work investigates how ribosome structure and function are affected by rRNA base modification. The prevailing view is that rRNA base modifications serve to fine tune ribosome structure and function.

Methodology/principal findings

To test this hypothesis, yeast strains deficient in rRNA modifications in the ribosomal peptidyltransferase center were monitored for changes in and translational fidelity. These studies revealed allele-specific sensitivity to translational inhibitors, changes in reading frame maintenance, nonsense

SUBMITTER: Baxter-Roshek JL 

PROVIDER: S-EPMC1766470 | biostudies-literature | 2007 Jan

REPOSITORIES: biostudies-literature