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A method for high-throughput gene expression signature analysis.

ABSTRACT: Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solution based on the combination of ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system.

SUBMITTER: Peck D

PROVIDER: S-EPMC1779561 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature

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A method for high-throughput gene expression signature analysis.

Peck David D Crawford Emily D ED Ross Kenneth N KN Stegmaier Kimberly K Golub Todd R TR Lamb Justin J

Genome biology 20060101 7

Genome-wide transcriptional profiling has shown that different biologic states (for instance, disease and response to pharmacologic manipulation) can be recognized by the expression pattern of relatively small numbers of genes. However, the lack of a practical and cost-effective technology for detection of these gene expression 'signatures' in large numbers of samples has severely limited their exploitation in important medical and pharmaceutical discovery applications. Here, we describe a solut ...[more]

PMID: 16859521

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