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Primate-specific evolution of an LDLR enhancer.

ABSTRACT: Sequence changes in regulatory regions have often been invoked to explain phenotypic divergence among species, but molecular examples of this have been difficult to obtain.In this study we identified an anthropoid primate-specific sequence element that contributed to the regulatory evolution of the low-density lipoprotein receptor. Using a combination of close and distant species genomic sequence comparisons coupled with in vivo and in vitro studies, we found that a functional cholesterol-sensing sequence motif arose and was fixed within a pre-existing enhancer in the common ancestor of anthropoid primates.Our study demonstrates one molecular mechanism by which ancestral mammalian regulatory elements can evolve to perform new functions in the primate lineage leading to human.

SUBMITTER: Wang QF

PROVIDER: S-EPMC1779597 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature

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Primate-specific evolution of an LDLR enhancer.

Wang Qian-fei QF Prabhakar Shyam S Wang Qianben Q Moses Alan M AM Chanan Sumita S Brown Myles M Eisen Michael B MB Cheng Jan-Fang JF Rubin Edward M EM Boffelli Dario D

Genome biology 20060802 8

<h4>Background</h4>Sequence changes in regulatory regions have often been invoked to explain phenotypic divergence among species, but molecular examples of this have been difficult to obtain.<h4>Results</h4>In this study we identified an anthropoid primate-specific sequence element that contributed to the regulatory evolution of the low-density lipoprotein receptor. Using a combination of close and distant species genomic sequence comparisons coupled with in vivo and in vitro studies, we found t ...[more]

PMID: 16884525

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