Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR.
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ABSTRACT:
SUBMITTER: Morey JS
PROVIDER: S-EPMC1779618 | biostudies-literature | 2006
REPOSITORIES: biostudies-literature
Publications
Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR.
Morey Jeanine S JS Ryan James C JC Van Dolah Frances M FM
Biological procedures online 20061212
Quantitative real-time PCR (qPCR) is a commonly used validation tool for confirming gene expression results obtained from microarray analysis; however, microarray and qPCR data often result in disagreement. The current study assesses factors contributing to the correlation between these methods in five separate experiments employing two-color 60-mer oligonucleotide microarrays and qPCR using SYBR green. Overall, significant correlation was observed between microarray and qPCR results (rho=0.708, ...[more]
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