Ontology highlight
ABSTRACT:
SUBMITTER: Garcia M
PROVIDER: S-EPMC1783778 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Garcia M M Darzacq X X Delaveau T T Jourdren L L Singer R H RH Jacq C C
Molecular biology of the cell 20061115 2
The coherence of mitochondrial biogenesis relies on spatiotemporally coordinated associations of 800-1000 proteins mostly encoded in the nuclear genome. We report the development of new quantitative analyses to assess the role of local protein translation in the construction of molecular complexes. We used real-time PCR to determine the cellular location of 112 mRNAs involved in seven mitochondrial complexes. Five typical cases were examined by an improved FISH protocol. The proteins produced in ...[more]