Ontology highlight
ABSTRACT:
SUBMITTER: Villen J
PROVIDER: S-EPMC1785252 | biostudies-literature | 2007 Jan
REPOSITORIES: biostudies-literature
Villén Judit J Beausoleil Sean A SA Gerber Scott A SA Gygi Steven P SP
Proceedings of the National Academy of Sciences of the United States of America 20070122 5
Protein phosphorylation is a complex network of signaling and regulatory events that affects virtually every cellular process. Our understanding of the nature of this network as a whole remains limited, largely because of an array of technical challenges in the isolation and high-throughput sequencing of phosphorylated species. In the present work, we demonstrate that a combination of tandem phosphopeptide enrichment methods, high performance MS, and optimized database search/data filtering stra ...[more]