Ontology highlight
ABSTRACT:
SUBMITTER: Lee HY
PROVIDER: S-EPMC1790958 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Lee Ho Young HY Khosla Chaitan C
PLoS biology 20070201 2
Macrolide antibiotics such as erythromycin are clinically important polyketide natural products. We have engineered a recombinant strain of Escherichia coli that produces small but measurable quantities of the bioactive macrolide 6-deoxyerythromycin D. Bioassay-guided evolution of this strain led to the identification of an antibiotic-overproducing mutation in the mycarose biosynthesis and transfer pathway that was detectable via a colony-based screening assay. This high-throughput assay was the ...[more]