PathogenMip assay: a multiplex pathogen detection assay.
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ABSTRACT:
SUBMITTER: Akhras MS
PROVIDER: S-EPMC1794193 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Publications
PathogenMip assay: a multiplex pathogen detection assay.
Akhras Michael S MS Thiyagarajan Sreedevi S Villablanca Andrea C AC Davis Ronald W RW Nyrén Pål P Pourmand Nader N
PloS one 20070221 2
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonstrated the utility of this assay with an initial set of 24 probes targeting the most clinically relevant HPV genotypes associated with cervical cancer progression. Probe construction was based on a novel, cost-effective, ligase-based protocol. The ass ...[more]
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