Unknown

Dataset Information

0

Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity.


ABSTRACT: Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these autoAbs were mainly IgG. The autoAbs were detectable 20 days after fever onset. Tests of non-SARS-pneumonia patients did not show the same autoAb production as in SARS patients. After sera IgG bound to A549 cells, cytotoxicity was induced. Cell cytotoxicity and the anti-epithelial cell IgG level were positively correlated. Preabsorption and binding assays indicated the existence of cross-reactive epitopes on SARS-CoV spike protein domain 2 (S2). Furthermore, treatment of A549 cells with anti-S2 Abs and IFN-gamma resulted in an increase in the adherence of human peripheral blood mononuclear cells to these epithelial cells. Taken together, we have demonstrated that the anti-S2 Abs in SARS patient sera cause cytotoxic injury as well as enhance immune cell adhesion to epithelial cells. The onset of autoimmune responses in SARS-CoV infection may be implicated in SARS pathogenesis.

SUBMITTER: Lin YS 

PROVIDER: S-EPMC1809466 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8099238 | biostudies-literature
| S-EPMC9354829 | biostudies-literature
| S-EPMC7670821 | biostudies-literature
| S-EPMC2906266 | biostudies-literature
| S-EPMC4054355 | biostudies-literature
| S-EPMC8008698 | biostudies-literature
| S-EPMC8058706 | biostudies-literature
| S-EPMC1489032 | biostudies-literature
| S-EPMC8070983 | biostudies-literature
| S-EPMC7323511 | biostudies-literature