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A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities.

ABSTRACT: A cantilever device based on competitive binding of an immobilized receptor to immobilized and soluble ligand and capable of measuring solution-phase thermodynamic quantities is described. Through multiple binary queries, the device stochastically measures the probability of the formation of a bound complex between immobilized protein and immobilized ligand as a function of soluble ligand concentration. The resulting binding isotherm is described by a binding polynomial consisting of the activities of soluble and immobilized ligand and binding constants for the association of immobilized protein with free and immobilized ligand. Evaluation of the polynomial reveals an association constant for the formation of a complex between immobilized ligand and immobilized protein close to that for the formation of complex between soluble protein and soluble ligand. The methodology lays the foundation for construction of practical portable sensing devices.

SUBMITTER: Snyder PW

PROVIDER: S-EPMC1815225 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature

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A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities.

Snyder Phillip W PW Lee Gwangrog G Marszalek Piotr E PE Clark Robert L RL Toone Eric J EJ

Proceedings of the National Academy of Sciences of the United States of America 20070216 8

A cantilever device based on competitive binding of an immobilized receptor to immobilized and soluble ligand and capable of measuring solution-phase thermodynamic quantities is described. Through multiple binary queries, the device stochastically measures the probability of the formation of a bound complex between immobilized protein and immobilized ligand as a function of soluble ligand concentration. The resulting binding isotherm is described by a binding polynomial consisting of the activit ...[more]

PMID: 17307881

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Project description:Previous studies, which examined whether symbolic and non-symbolic quantity representations are processed by two independent systems or by one common system, reached contradicting findings, possibly due to methodological differences. Indeed, some researchers advocate the two systems approach, based on the presence of notation-specific switch cost in conditions where adults have to compare pairs of symbolic and non-symbolic quantities, in combination with the absence of such a cost in conditions containing quantities of the same notation. However, other researchers used matching instructions, and reported a facilitation in the mixed notation conditions, suggesting that the two systems are automatically integrated. In the current study, we conducted three experiments, in which we examined the existence of two separate quantity systems, but we used various experimental manipulations (e.g., task instructions, presentation order) to unravel the previous inconsistent findings. In Experiment 1, we investigated the role of task instructions by presenting participants with pure and mixed notation trials with both comparison and matching tasks. In Experiment 2, we tested the role of blocked and randomized presentation order for the pure and mixed trials. Our data showed that cost for switching between the symbolic and non-symbolic quantities is present, but is prone to a certain methodological drawback: when the differences between the processing times for two sequentially presented stimuli of different notations are not taken into account, this masks the cost for switching between the two systems. To overcome this problem, in Experiment 3 we used an audio-visual paradigm. Overall, our results provide further evidence for the existence of distinct quantity representations, independently of task instructions or presentation order. Additionally, considering this methodological pitfall we argue that the audio-visual paradigm is better suited when investigating the integration between symbolic and non- symbolic quantities.

Dataset Information

A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities.

Publications

A stochastic, cantilever approach to the evaluation of solution phase thermodynamic quantities.

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