Ontology highlight
ABSTRACT:
SUBMITTER: Polycarpo CR
PROVIDER: S-EPMC1817836 | biostudies-literature | 2006 Dec
REPOSITORIES: biostudies-literature
Polycarpo Carla R CR Herring Stephanie S Bérubé Amélie A Wood John L JL Söll Dieter D Ambrogelly Alexandre A
FEBS letters 20061120 28-29
In certain methanogenic archaea a new amino acid, pyrrolysine (Pyl), is inserted at in-frame UAG codons in the mRNAs of some methyltransferases. Pyl is directly acylated onto a suppressor tRNA(Pyl) by pyrrolysyl-tRNA synthetase (PylRS). Due to the lack of a readily available Pyl source, we looked for structural analogues that could be aminoacylated by PylRS onto tRNA(Pyl). We report here the in vitro aminoacylation of tRNA(Pyl) by PylRS with two Pyl analogues: N-epsilon-d-prolyl-l-lysine (d-prol ...[more]