Ontology highlight
ABSTRACT:
SUBMITTER: Robine N
PROVIDER: S-EPMC1820458 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
Robine Nicolas N Uematsu Norio N Amiot Franck F Gidrol Xavier X Barillot Emmanuel E Nicolas Alain A Borde Valérie V
Molecular and cellular biology 20061222 5
Meiotic recombination is initiated by the formation of programmed DNA double-strand breaks (DSBs) catalyzed by the Spo11 protein. DSBs are not randomly distributed along chromosomes. To better understand factors that control the distribution of DSBs in budding yeast, we have examined the genome-wide binding and cleavage properties of the Gal4 DNA binding domain (Gal4BD)-Spo11 fusion protein. We found that Gal4BD-Spo11 cleaves only a subset of its binding sites, indicating that the association of ...[more]